Biopolymer encapsulated live influenza virus as a universal CD8+ T cell vaccine against influenza virus

Vaccine. 2010 Dec 16;29(2):314-22. doi: 10.1016/j.vaccine.2010.10.036. Epub 2010 Oct 27.

Abstract

Current influenza virus vaccines primarily elicit antibodies and can be rendered ineffective by antigenic drift and shift. Vaccines that elicit CD8+ T cell responses targeting less variable proteins may function as universal vaccines that have broad reactivity against different influenza virus strains. To generate such a universal vaccine, we encapsulated live influenza virus in a biopolymer and delivered it to mice subcutaneously. This vaccine was safe, induced potent CD8+ T cell immunity and protected mice against heterosubtypic lethal challenge. Safety of subcutaneous (SQ) vaccination was tested in Rag-/-γc-/- double knockout mice which we show cannot control intranasal infection. Biopolymer encapsulation of live influenza virus could be used to develop universal CD8+ T cell vaccines against heterosubtypic and pandemic strains.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biopolymers / administration & dosage*
  • Disease Models, Animal
  • Drug Carriers / administration & dosage*
  • Female
  • Fetal Proteins
  • Influenza Vaccines / administration & dosage
  • Influenza Vaccines / adverse effects
  • Influenza Vaccines / immunology*
  • Injections, Subcutaneous
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Orthomyxoviridae / immunology*
  • Orthomyxoviridae Infections / prevention & control
  • T-Box Domain Proteins
  • T-Lymphocytes / immunology*
  • Vaccines

Substances

  • Biopolymers
  • Drug Carriers
  • Fetal Proteins
  • Influenza Vaccines
  • T-Box Domain Proteins
  • Vaccines
  • Brachyury protein