Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR

Yu Chen; Dawei Cui; Shufa Zheng; Shigui Yang; Jia Tong; Dagan Yang; Jian Fan; Jie Zhang; Bin Lou; Xuefen Li; Xiaoling Zhuge; Bo Ye; Baode Chen; Weilin Mao; Yajun Tan; Genyun Xu; Zhenjin Chen; Nan Chen; Lanjuan Li

doi:10.1128/JCM.02184-10

Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR

J Clin Microbiol. 2011 Apr;49(4):1653-6. doi: 10.1128/JCM.02184-10. Epub 2011 Jan 26.

Authors

Affiliation

¹ Center of Clinical Laboratory, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Abstract

A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Clinical Laboratory Techniques / methods*
Humans
Influenza A Virus, H1N1 Subtype / isolation & purification
Influenza A Virus, H3N2 Subtype / isolation & purification
Influenza B virus / isolation & purification
Influenza, Human / diagnosis*
Influenza, Human / virology*
Polymerase Chain Reaction / methods*
Respiratory Syncytial Virus Infections / diagnosis*
Respiratory Syncytial Virus Infections / virology*
Respiratory Syncytial Viruses / isolation & purification
Sensitivity and Specificity
Virology / methods*