Validation and diagnostic application of NS and HA gene-specific real-time reverse transcription-PCR assays for detection of 2009 pandemic influenza A (H1N1) viruses in clinical specimens

Esa Rönkkö; Niina Ikonen; Mia Kontio; Minna Haanpää; Hannimari Kallio-Kokko; Laura Mannonen; Maija Lappalainen; Ilkka Julkunen; Thedi Ziegler

doi:10.1128/JCM.00259-11

Validation and diagnostic application of NS and HA gene-specific real-time reverse transcription-PCR assays for detection of 2009 pandemic influenza A (H1N1) viruses in clinical specimens

J Clin Microbiol. 2011 May;49(5):2009-11. doi: 10.1128/JCM.00259-11. Epub 2011 Mar 2.

Authors

Esa Rönkkö¹, Niina Ikonen, Mia Kontio, Minna Haanpää, Hannimari Kallio-Kokko, Laura Mannonen, Maija Lappalainen, Ilkka Julkunen, Thedi Ziegler

Affiliation

¹ Viral Infections Unit, Department of Vaccination and Immune Protection, National Institute for Health and Welfare (THL),Helsinki, Finland. esa.ronkko@thl.fi

Abstract

Real-time reverse transcription-PCR assays specific for the nonstructural (NS) and hemagglutinin (HA) genes of the 2009 pandemic influenza A (H1N1) virus were developed and evaluated with clinical samples from infected patients. The tests are characterized by high sensitivity and specificity and performed well throughout the first year of the 2009 pandemic.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Hemagglutinin Glycoproteins, Influenza Virus / genetics*
Humans
Influenza A Virus, H1N1 Subtype / genetics
Influenza A Virus, H1N1 Subtype / isolation & purification*
Influenza, Human / diagnosis*
Influenza, Human / virology*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Viral Nonstructural Proteins / genetics*
Virology / methods*

Substances

Hemagglutinin Glycoproteins, Influenza Virus
NS protein, influenza virus
Viral Nonstructural Proteins