Abstract
The candidate pandemic H1N1 vaccine virus NIBRG-121 was derived by reverse genetics and comprises the hemagglutinin (HA) and neuraminidase (NA) genes from A/California/7/2009 (CAL) on an A/Puerto Rico/8/34 (PR8) backbone. NIBRG-121 was found to grow poorly in eggs, compared to seasonal H1N1 candidate vaccine viruses. Based on our previous study with H5N1 candidate vaccine viruses, we generated two new viruses with chimeric PR8/CAL HA genes. Here we show that these new viruses have considerably improved growth in eggs and are therefore better candidate vaccine viruses for use in production of pandemic H1N1 (2009) vaccine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chick Embryo
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology
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Hemagglutinin Glycoproteins, Influenza Virus / metabolism*
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Humans
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Influenza A Virus, H1N1 Subtype / genetics
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Influenza A Virus, H1N1 Subtype / growth & development*
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Influenza A Virus, H1N1 Subtype / immunology
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Influenza A virus / genetics*
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Influenza A virus / metabolism
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Influenza Vaccines*
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Influenza, Human / prevention & control
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Molecular Sequence Data
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Neuraminidase / chemistry
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Neuraminidase / genetics
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Neuraminidase / immunology
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Neuraminidase / metabolism*
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Pandemics*
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / metabolism*
Substances
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Hemagglutinin Glycoproteins, Influenza Virus
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Influenza Vaccines
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Recombinant Proteins
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Neuraminidase