Abstract
Despite existing vaccines and specific therapies, epidemics of seasonal influenza annually claim 200,000-500,000 lives worldwide. Pandemic influenza represents an even greater threat, with numerous potentially pandemic viruses circulating in nature. Development of multi-specific vaccines against multiple pandemic or seasonal strains is important for human health and the global economy. Here we report a novel virus-like particle (VLP) platform that contains three hemagglutinin (HA) subtypes. This recombinant vaccine design resulted in the expression of three HA subtypes co-localized within a VLP. Experimental triple-HA VLPs containing HA proteins derived from H5N1, H7N2, and H2N3 viruses were immunogenic and protected ferrets from challenge from all three potentially pandemic viruses. Similarly, VLPs containing HA subtypes derived from seasonal H1N1, H3N2, and type B influenza viruses protected ferrets from three seasonal influenza viruses. We conclude that this technology may represent a novel strategy for rapid development of trivalent seasonal and pandemic vaccines.
Published by Elsevier Ltd.
MeSH terms
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Animals
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Cells, Cultured
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Ferrets
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology*
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Hemagglutinin Glycoproteins, Influenza Virus / metabolism
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Humans
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Influenza A Virus, H1N1 Subtype / genetics
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Influenza A Virus, H1N1 Subtype / immunology
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Influenza A Virus, H3N2 Subtype / genetics
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Influenza A Virus, H3N2 Subtype / immunology
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Influenza A Virus, H5N1 Subtype / genetics
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Influenza A Virus, H5N1 Subtype / immunology
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Influenza A Virus, H7N2 Subtype / genetics
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Influenza A Virus, H7N2 Subtype / immunology
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Influenza B virus / genetics
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Influenza B virus / immunology
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / genetics
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Influenza Vaccines / immunology*
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Influenza, Human / immunology
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Influenza, Human / prevention & control
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Influenza, Human / virology
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Orthomyxoviridae / classification
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Orthomyxoviridae / genetics
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Orthomyxoviridae / immunology*
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Orthomyxoviridae Infections / immunology
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Orthomyxoviridae Infections / prevention & control*
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Orthomyxoviridae Infections / virology
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Pandemics
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Seasons
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Spodoptera
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology*
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Virion / genetics
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Virion / immunology*
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Virion / metabolism
Substances
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Hemagglutinin Glycoproteins, Influenza Virus
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Influenza Vaccines
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Vaccines, Synthetic