Retrospective and prospective verification of the Cepheid Xpert influenza virus assay

Elena B Popowitch; Elaine Rogers; Melissa B Miller

doi:10.1128/JCM.01162-11

Retrospective and prospective verification of the Cepheid Xpert influenza virus assay

J Clin Microbiol. 2011 Sep;49(9):3368-9. doi: 10.1128/JCM.01162-11. Epub 2011 Jul 20.

Authors

Elena B Popowitch¹, Elaine Rogers, Melissa B Miller

Affiliation

¹ UNC School of Medicine, Department of Pathology and Laboratory Medicine, Campus Box 7525, Chapel Hill, NC 27599-7525, USA.

Abstract

We performed a retrospective (n = 121) and prospective (n = 305) verification of the Cepheid Xpert Flu assay to determine its performance characteristics. The overall sensitivity and specificity were 93% and 100%, respectively. Nasopharyngeal specimen sensitivities were 100% for seasonal influenza A/H1 virus and influenza A/H3 virus, 90% for influenza A/2009/H1N1 virus, and 95% for influenza B virus.

Publication types

Evaluation Study

MeSH terms

Humans
Influenza A virus / classification
Influenza A virus / isolation & purification*
Influenza B virus / classification
Influenza B virus / isolation & purification*
Influenza, Human / diagnosis*
Influenza, Human / virology*
Molecular Diagnostic Techniques / methods*
Nasopharynx / virology
Prospective Studies
Retrospective Studies
Sensitivity and Specificity
Virology / methods*