Stability of seasonal influenza vaccines investigated by spectroscopy and microscopy methods

E Patois; M A H Capelle; R Gurny; T Arvinte

doi:10.1016/j.vaccine.2011.07.067

Stability of seasonal influenza vaccines investigated by spectroscopy and microscopy methods

Vaccine. 2011 Oct 6;29(43):7404-13. doi: 10.1016/j.vaccine.2011.07.067. Epub 2011 Jul 29.

Authors

E Patois¹, M A H Capelle, R Gurny, T Arvinte

Affiliation

¹ School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland.

PMID: 21803109
DOI: 10.1016/j.vaccine.2011.07.067

Abstract

The stability of different seasonal influenza vaccines was investigated by spectroscopy and microscopy methods before and after the following stress-conditions: (i) 2 and 4 weeks storage at 25°C, (ii) 1 day storage at 37°C and (iii) one freeze-thaw cycle. The subunit vaccine Influvac (Solvay Pharma) and the split vaccine Mutagrip (Sanofi Pasteur) were affected by all stresses. The split vaccine Fluarix (GlaxoSmithKline) was affected only by storage at 25°C. The virosomal vaccine Inflexal V (Berna Biotech) was stable after the temperature stresses but aggregated after one freeze-thaw cycle. This study provides new insights into commercial vaccines of low antigen concentration and highlights the importance of using multiple techniques to assess vaccine stability.

MeSH terms

Drug Stability*
Drug Storage*
Influenza Vaccines*
Microscopy, Fluorescence
Spectrometry, Fluorescence
Spectrum Analysis
Temperature
Vaccines, Subunit

Substances

Influenza Vaccines
Vaccines, Subunit