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Articles published in J Virol Methods

Retrieve available abstracts of 82 articles:
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Single Articles


    December 2025
  1. GONZALEZ G S, Dias A, Reis R, Villalba S, et al
    Effective RNA extraction with readily available reagents: A comparative analysis of alternative protocols.
    J Virol Methods. 2025;338:115242.
    PubMed     Abstract available


  2. ZENG L, Wang Y, Xiang B, Lin J, et al
    The dynamics of common respiratory pathogens in southern china under the context of COVID-19 pandemic and prediction of mycoplasma pneumonia (2018-2023).
    J Virol Methods. 2025;338:115240.
    PubMed     Abstract available


  3. KATSANOVSKAJA K, Marchesin F, Ujetz J, Ijaz S, et al
    Beyond the limits of conventional 'endpoint' ELISA and rescuing the signal with lag k-ELISA.
    J Virol Methods. 2025;338:115231.
    PubMed     Abstract available


  4. MORRISON EN, Harnden M, Boisvert E, Wilson AE, et al
    Towards efficient and targeted sampling of primary respiratory diseases from wastewater in congregate settings for seniors: Empowering high-risk demographics with prospective health threat data.
    J Virol Methods. 2025;338:115212.
    PubMed     Abstract available


  5. LESCHINSKY G, Dexter WA, Leschinsky B, Trolinger J, et al
    Design and feasibility of a novel personal protection device against airborne pathogens for everyday use.
    J Virol Methods. 2025;338:115203.
    PubMed     Abstract available


  6. WAGNER DD, Nabakooza G, Momin N, Marine RL, et al
    Illumina MiSeq and iSeq platforms yield comparable results for viral genomic sequencing.
    J Virol Methods. 2025;338:115202.
    PubMed     Abstract available


  7. LIM JY, Fiore A, Le B, Minzer C, et al
    Development and Validation of Novel Cell-free Direct Neutralization Assay for SARS-CoV-2.
    J Virol Methods. 2025;338:115201.
    PubMed     Abstract available


    September 2025
  8. NAPOLITAN A, Mazzacan E, Fonti N, Tomasoni S, et al
    Cryopreservation of chicken and duck tracheal rings and precision-cut lung slices: A promising tool for the rapid characterization of avian influenza viruses.
    J Virol Methods. 2025;339:115257.
    PubMed     Abstract available


  9. CHENTOUFI HA, Galipeau Y, Arnold C, Dewar-Darch D, et al
    Comparative performance of serum and plasma samples in SARS-CoV-2 serology and neutralization assays.
    J Virol Methods. 2025;337:115186.
    PubMed     Abstract available


  10. COLOTTE M, Luis A, Coudy D, Tuffet S, et al
    Room temperature storage and shipping of encapsulated synthetic RNAs as quality control materials for SARS-CoV-2 molecular diagnostic assays.
    J Virol Methods. 2025;337:115169.
    PubMed     Abstract available


    August 2025
  11. MAROTTA MG, Neto MM, Daly J, Maina M, et al
    Development and optimisation of Influenza C and Influenza D pseudotyped viruses.
    J Virol Methods. 2025 Aug 23:115243. doi: 10.1016/j.jviromet.2025.115243.
    PubMed     Abstract available


  12. WANG P, Yan L, Wang J, Hu S, et al
    Utilizing multiplex reverse transcription-multiple cross displacement amplification-lateral flow biosensor technology for detecting H1N1, H3N2 and H7N9 influenza A virus subtypes.
    J Virol Methods. 2025;338:115235.
    PubMed     Abstract available


    July 2025
  13. PUGLIA I, Caporale M, Di Teodoro G, Spedicato M, et al
    Optimization of an infectious subgenomic amplicons reverse genetics protocol for the rescue of synthetic coronaviruses.
    J Virol Methods. 2025;336:115152.
    PubMed     Abstract available


    June 2025
  14. CHANG C, Li L, Guo Y, Ji L, et al
    The potential application of digital PCR in detecting different SARS-CoV-2 viral loads.
    J Virol Methods. 2025;335:115151.
    PubMed     Abstract available


  15. DE BEER C, Vanmali HD
    Genotypic analysis of rhinovirus and human respiratory syncytial virus in sudden unexpected death in infancy cases at Tygerberg Hospital, Cape Town, South Africa.
    J Virol Methods. 2025;335:115150.
    PubMed     Abstract available


  16. ZARGAR B, Sattar SA, McKinney J, Ijaz MK, et al
    The stability and elimination of mammalian enveloped and non-enveloped respiratory and enteric viruses in indoor air: Testing using a room-sized aerobiology chamber.
    J Virol Methods. 2025;335:115144.
    PubMed     Abstract available


  17. REYNA RA, Walker J, Viveros A, Mitchell B, et al
    Optimization of a panel of behavioral tests for use in containment using a golden Syrian hamster model.
    J Virol Methods. 2025;335:115132.
    PubMed     Abstract available


    May 2025
  18. GASTELBONDO-PASTRANA B, Florez L, Guzman C, Torres K, et al
    Phenol-free in-house kit for RNA extraction with applicability to SARS-CoV-2 genomic sequencing studies: A contribution to biotechnological sovereignty in Colombia.
    J Virol Methods. 2025;334:115116.
    PubMed     Abstract available


    April 2025
  19. WU JS, Kan JY, Chang YS, Phuong Le UN, et al
    Developing Zika virus-transduced hACE2 expression models for severe acute respiratory syndrome coronavirus 2 infection in vitro and in vivo.
    J Virol Methods. 2025 Apr 14:115166. doi: 10.1016/j.jviromet.2025.115166.
    PubMed     Abstract available


  20. NGUYEN MA, Williams C, Gard AL, Connor JH, et al
    Endothelial growth media components alter SARS-CoV-2 spike-directed growth kinetics.
    J Virol Methods. 2025;333:115111.
    PubMed     Abstract available


  21. FRAISSE A, Guillier L, Cordevant C, Le Poder S, et al
    Impedance-based method for the quantification of infectious SARS-CoV-2.
    J Virol Methods. 2025;333:115110.
    PubMed     Abstract available


  22. HOFF K, Ding X, Liu XL, Lin JY, et al
    Rapid, inexpensive multiplex pathogen detection using resequencing microarrays.
    J Virol Methods. 2025;333:115109.
    PubMed     Abstract available


  23. LOPEZ KP, Cool KR, Bold D, Gaudreault NN, et al
    Detection of SARS-CoV-2- specific antibodies in domestic cats using different ELISA tests.
    J Virol Methods. 2025;333:115099.
    PubMed     Abstract available


    February 2025
  24. IBRAHIM S, Spackman E, Suarez DL, Goraichuk IV, et al
    Evaluation of an N1 NA antibody-specific enzyme-linked lectin assay for detection of H5N1 highly pathogenic avian influenza virus infection in vaccinated birds.
    J Virol Methods. 2025 Feb 14:115127. doi: 10.1016/j.jviromet.2025.115127.
    PubMed     Abstract available


  25. ASHLEY CL, Bloul M, Alca S, Smith L, et al
    Optimisation of a multiplexed, high throughput assay to measure neutralising antibodies against SARS-CoV-2 variants.
    J Virol Methods. 2025;332:115073.
    PubMed     Abstract available


  26. DA SILVA JG, Arruk VG, Veiga GRLD, Sousa LVA, et al
    Quantitative and qualitative analysis of seroconversion after one year of vaccination with inactivated SARS-CoV-2 vaccine (CoronaVac(R)) in healthcare workers: Cross-sectional analytical study.
    J Virol Methods. 2025;332:115067.
    PubMed     Abstract available


    January 2025
  27. FIBRIANI A, Naisanu K, Yamahoki N, Kinanti DR, et al
    Development of polyclonal chicken egg yolk immunoglobulin Y (IgY) antibodies targeting SARS-CoV-2 multi-epitope antigen.
    J Virol Methods. 2025;331:115062.
    PubMed     Abstract available


  28. YAMAZAKI Y, Tanaka R, Castillo G, Macalanda AMC, et al
    Development of a concentration method for simple and sensitive detection of SARS-CoV-2 in saliva using a magnetic nanoparticle kit.
    J Virol Methods. 2025;331:115059.
    PubMed     Abstract available


  29. MDUNYELWA A, Seema C, Mabaso A, Mlambo K, et al
    Evaluation of the Seegene Allplex RV master assay for one-step simultaneous detection of eight respiratory viruses in nasopharyngeal specimens.
    J Virol Methods. 2025;331:115042.
    PubMed     Abstract available


    December 2024
  30. YANG H, Fei C, Wang S, Shen X, et al
    Validation of an HPLC-CAD method for measuring the lipid content of novel LNP-encapsulated COVID-19 mRNA vaccines.
    J Virol Methods. 2024;330:115040.
    PubMed     Abstract available


  31. HLEKELELE L, Setshedi K, Mandiwana V, Kalombo L, et al
    Carboxy-PEG-thiol functionalized gold nanoparticle conjugates for the detection of SARS-CoV-2: Detection tools and analytical method development.
    J Virol Methods. 2024;330:115028.
    PubMed     Abstract available


  32. KULKARNI PM, Basagoudanavar SH, Gopinath S, Patangia H, et al
    Characterization of monoclonal antibodies targeting SARS-CoV-2 spike glycoprotein: Reactivity against Delta and Omicron BA.1 variants.
    J Virol Methods. 2024;330:115027.
    PubMed     Abstract available


  33. ALATHARI MJA, Mashhadany YA, Bakar AAA, Mokhtar MHH, et al
    COVID-19 IgG antibodies detection based on CNN-BiLSTM algorithm combined with fiber-optic dataset.
    J Virol Methods. 2024;330:115011.
    PubMed     Abstract available


    September 2024
  34. ZHANG Y, Kong W, Wu Y, Chen Z, et al
    Reducing amplification cycles to improve the coverage of influenza A virus genome sequencing in heterosubtypic co-infection.
    J Virol Methods. 2024;330:115036.
    PubMed     Abstract available


  35. FUTSCHIK ME, Tunkel SA, Turek E, Chapman D, et al
    Double testing with lateral flow antigen test devices for COVID-19: does a second test in quick succession add value?
    J Virol Methods. 2024;329:115000.
    PubMed     Abstract available


  36. SINGH A, Sahu U, Kulkarni PM, Yadav R, et al
    A safe, cost-effective, and high-throughput SARS-CoV-2 antigen capture ELISA suitable for large-scale screening in low-resource settings.
    J Virol Methods. 2024;329:114995.
    PubMed     Abstract available


  37. FOGACA MBT, Lopes-Luz L, Saavedra DP, de Oliveira NKAB, et al
    Production of antigens expressed in Nicotiana benthamiana plant and Escherichia coli for the SARS-CoV-2 IgG antibody detection by ELISA.
    J Virol Methods. 2024;329:114969.
    PubMed     Abstract available


    July 2024
  38. MORECCHIATO F, Coppi M, Niccolai C, Antonelli A, et al
    Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples.
    J Virol Methods. 2024;328:114956.
    PubMed     Abstract available


    June 2024
  39. MAK WA, Visser W, Koeleman JGM, Ong DSY, et al
    SARS-CoV-2-specific T cell responses: a comparative analysis between QuantiFERON SARS-CoV-2, T-SPOT.COVID, and an in-house Omicron ELISpot.
    J Virol Methods. 2024;327:114949.
    PubMed     Abstract available


  40. HAMILTON S, Zhu M, Cloney-Clark S, Mayes P, et al
    Validation of a severe acute respiratory syndrome coronavirus 2 microneutralization assay for evaluation of vaccine immunogenicity.
    J Virol Methods. 2024;327:114945.
    PubMed     Abstract available


  41. EDWARDS K, Corocher T, Hersusianto Y, Campbell D, et al
    Heparin-mediated PCR interference in SARS-CoV-2 assays and subsequent reversal with heparinase I.
    J Virol Methods. 2024;327:114944.
    PubMed     Abstract available


  42. VASQUEZ V, Orozco J
    Clinical validation of SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex.
    J Virol Methods. 2024;327:114940.
    PubMed     Abstract available


  43. DOSBAA A, Guilbaud R, Yusti AF, Ferre VM, et al
    RSV-GenoScan: An automated pipeline for whole-genome human respiratory syncytial virus (RSV) sequence analysis.
    J Virol Methods. 2024;327:114938.
    PubMed     Abstract available


  44. OUSSET MJ, Pianciola LA, Mazzeo M, Oteiza JM, et al
    Improved SARS-CoV-2 RNA recovery in wastewater matrices using a CTAB-based extraction method.
    J Virol Methods. 2024;327:114918.
    PubMed     Abstract available


    May 2024
  45. ZHOU H, Li H, Sun X, Lin J, et al
    Rapid diagnosis of Canine respiratory coronavirus, Canine influenza virus, Canine distemper virus and Canine parainfluenza virus with a Taqman probe-based multiplex real-time PCR.
    J Virol Methods. 2024 May 30:114960. doi: 10.1016/j.jviromet.2024.114960.
    PubMed     Abstract available


  46. BOESELT T, Terhorst P, Kroenig J, Nell C, et al
    Specific molecular peak analysis by ion mobility spectrometry of volatile organic compounds in urine of COVID-19 patients: A novel diagnostic approach.
    J Virol Methods. 2024;326:114910.
    PubMed     Abstract available


  47. KATSUNO T, Kimura M, Terada-Hirashima J, Kazuyama Y, et al
    Diagnostic accuracy of direct reverse transcription-polymerase chain reaction using guanidine-based and guanidine-free inactivators for SARS-CoV-2 detection in saliva samples.
    J Virol Methods. 2024;326:114909.
    PubMed     Abstract available


  48. BANG LL, Tornby DR, Pham STD, Assing K, et al
    Culturing of SARS-CoV-2 from patient samples: Protocol for optimal virus recovery and assessment of infectious viral load.
    J Virol Methods. 2024;326:114912.
    PubMed     Abstract available


  49. LEE SS, Kim AL, Park JH, Lee DH, et al
    Optimization of duplex digital PCR for the measurement of SARS-CoV-2 RNA.
    J Virol Methods. 2024;326:114911.
    PubMed     Abstract available


  50. FERNANDEZ-SANCHEZ F, Martin-Bautista E, Rivas-Ruiz F, Wu W, et al
    Evaluation of the EasyNAT SARS-CoV-2 assay PCR test for the diagnosis of SARS-CoV-2 infection.
    J Virol Methods. 2024;326:114908.
    PubMed     Abstract available


  51. SUZUKI R, Kamiyama A, Ito H, Kawashiro K, et al
    The development of a rapid, high-throughput neutralization assay using a SARS-CoV-2 reporter.
    J Virol Methods. 2024;326:114894.
    PubMed     Abstract available


    April 2024
  52. KRUNKOSKY M, Krunkosky TM, Meliopoulos V, Kyriakis CS, et al
    Establishment of Swine Primary Nasal, Tracheal, and Bronchial Epithelial Cell Culture Models for the Study of Influenza Virus Infection.
    J Virol Methods. 2024 Apr 26:114943. doi: 10.1016/j.jviromet.2024.114943.
    PubMed     Abstract available


  53. AN SH, Kim NY, Heo GB, Kang YM, et al
    Development and evaluation of a multiplex real-time RT-PCR assay for simultaneous detection of H5, H7, and H9 subtype avian influenza viruses.
    J Virol Methods. 2024;327:114942.
    PubMed     Abstract available


    February 2024
  54. POURAKBARI R, Gholami M, Shakerimoghaddam A, Khiavi FM, et al
    Comparison of RT-LAMP and RT-qPCR assays for detecting SARS-CoV-2 in the extracted RNA and direct swab samples.
    J Virol Methods. 2024;324:114871.
    PubMed     Abstract available


    January 2024
  55. VAN SLAMBROUCK J, Schoenaers C, Laenen L, Jin X, et al
    The value of point-of-care tests for the detection of SARS-CoV-2 RNA or antigen in bronchoalveolar lavage fluid.
    J Virol Methods. 2024;323:114848.
    PubMed     Abstract available


  56. WANI AK, Chopra C, Dhanjal DS, Akhtar N, et al
    Metagenomics in the fight against zoonotic viral infections: A focus on SARS-CoV-2 analogues.
    J Virol Methods. 2024;323:114837.
    PubMed     Abstract available


    December 2023
  57. ELFAYRES G, Paswan RR, Sika L, Girard MP, et al
    Mammalian cells-based platforms for the generation of SARS-CoV-2 virus-like particles.
    J Virol Methods. 2023;322:114835.
    PubMed     Abstract available


  58. LOWRY K, Bauer MJ, Buckley C, Wang C, et al
    Evaluation of Illumina(R) COVIDSeq as a tool for Omicron SARS-CoV-2 characterisation.
    J Virol Methods. 2023;322:114827.
    PubMed     Abstract available


  59. MOGLING R, Reimerink J, Stanoeva KR, Keramarou M, et al
    Comparative study between virus neutralisation testing and other serological methods detecting anti-SARS-CoV-2 antibodies in Europe, 2021.
    J Virol Methods. 2023;322:114825.
    PubMed     Abstract available


    November 2023
  60. BOUJEMAA S, Suri GS, Kaur G
    Diagnostic evaluation of Panbio antigen rapid diagnostic test for SARS-CoV-2: A systematic review and meta-analysis.
    J Virol Methods. 2023;321:114811.
    PubMed     Abstract available


  61. JASSOY C, Biemann R, Honemann M
    Low BAU/ml values with 4 of 5 SARS CoV-2 spike-specific monoclonal antibodies in the Roche Elecsys antibody assay.
    J Virol Methods. 2023;321:114786.
    PubMed     Abstract available


    October 2023
  62. AMANO K, Kurebayashi Y, Takahashi T, Narimichi Y, et al
    Visualizing intracellular sialidase activity of influenza A virus neuraminidase using a fluorescence imaging probe.
    J Virol Methods. 2023 Oct 30:114838. doi: 10.1016/j.jviromet.2023.114838.
    PubMed     Abstract available


  63. DI DYW, Li B, Jeon MK, Yan T, et al
    Comparing solid-based concentration methods for rapid and efficient recovery of SARS-CoV-2 for wastewater surveillance.
    J Virol Methods. 2023;320:114790.
    PubMed     Abstract available


  64. DERIN DC, Gultekin E, Taskin II, Otlu B, et al
    Designing of rapid assay for the detection of RdRp/Orf1ab specific to SARS-CoV-2.
    J Virol Methods. 2023;320:114774.
    PubMed     Abstract available


    September 2023
  65. PAWAR SD, Tare DS, Kode SS, Keng SS, et al
    Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage.
    J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830.
    PubMed     Abstract available


  66. FLINCK H, Aittoniemi J
    Evaluation of the new 2.0 version of the Roche SARS-CoV-2 Rapid Antigen Test.
    J Virol Methods. 2023;319:114758.
    PubMed     Abstract available


  67. EPTAMINITAKI GC, Parakatselaki ME, Petroulaki C, Marinopoulou D, et al
    Rapid identification of SARS-CoV-2 variants: Validation of the simplexa SARS-CoV-2 variant direct assay.
    J Virol Methods. 2023;319:114759.
    PubMed     Abstract available


    July 2023
  68. NOH EB, Heo GB, Lee KN, Kang YM, et al
    Subtype specific virus enrichement with immunomagnetic separation method followed by NGS unravels the mixture of H5 and H9 avian influenza virus.
    J Virol Methods. 2023;320:114773.
    PubMed     Abstract available


  69. DE SOUZA ANDRADE A, Freitas EF, de Castro Barbosa E, Guimaraes NR, et al
    Potential use of high-resolution melting analyses for SARS-CoV-2 genomic surveillance.
    J Virol Methods. 2023;317:114742.
    PubMed     Abstract available


  70. AHMED W, Smith WJM, Sirikanchana K, Kitajima M, et al
    Influence of membrane pore-size on the recovery of endogenous viruses from wastewater using an adsorption-extraction method.
    J Virol Methods. 2023;317:114732.
    PubMed     Abstract available


  71. PILCHOVA V, Prajeeth CK, Jendrny P, Twele F, et al
    beta-Propiolactone (BPL)-inactivation of SARS-Co-V-2: In vitro validation with focus on saliva from COVID-19 patients for scent dog training.
    J Virol Methods. 2023;317:114733.
    PubMed     Abstract available


    June 2023
  72. LAMOTTE LA, Tafforeau L
    Generation of an A549 ISRE-luciferase stable cell line.
    J Virol Methods. 2023;316:114731.
    PubMed     Abstract available


  73. FILIPPATOS F, Tatsi EB, Papagiannopoulos C, Syriopoulou V, et al
    Comparison of a rapid fluorescence immunochromatographic test with an enzyme-linked immunosorbent assay for measurement of SARS-CoV-2 spike protein antibody neutralizing activity.
    J Virol Methods. 2023;316:114728.
    PubMed     Abstract available


  74. HEAPS AL, Sobolewski MD, Jacobs JL, Gordon KC, et al
    Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer.
    J Virol Methods. 2023;316:114726.
    PubMed     Abstract available


  75. ABD-ELDAIM MM, Maarouf M, Potgieter L, Kania SA, et al
    Amino acid variations of the immuno-dominant domain of respiratory syncytial virus attachment glycoprotein (G) affect the antibody responses In BALB/c mice.
    J Virol Methods. 2023;316:114712.
    PubMed     Abstract available


  76. CROWE A, Lorenzo YP, Jardine D, Visvanathan K, et al
    Evaluation of a novel anterior nasal swab for the detection of SARS-CoV-2.
    J Virol Methods. 2023;316:114713.
    PubMed     Abstract available


  77. EFFAH K, Agbemafle I, Essel NO, Amuah JE, et al
    Comparison of the AmpFire and MA-6000 polymerase chain reaction platforms for high-risk human papillomavirus testing in cervical precancer screening.
    J Virol Methods. 2023;316:114709.
    PubMed     Abstract available


    May 2023
  78. DAKHAVE M, Gadekar S, Malekar A, Wankhede G, et al
    'CoviSwift(TM'): A point-of-care RT-PCR device for SARS-CoV-2 and its variant detection.
    J Virol Methods. 2023;315:114714.
    PubMed     Abstract available


  79. ANDRADE IA, Franca LV, Kauffmann CM, Maeda MHK, et al
    Practical use of tobravirus-based vector to produce SARS-CoV-2 antigens in plants.
    J Virol Methods. 2023;315:114710.
    PubMed     Abstract available


  80. SANCHEZ-PACHECO UA, Bahena-Mondragon BM, Hernandez-Piedras FR, Soria-Osorio R, et al
    Development of a validated molecular analytical method to determine the viral safety of F(AB )(2) products: A novel application for a well-known technique.
    J Virol Methods. 2023;315:114694.
    PubMed     Abstract available


    April 2023
  81. KAMI W, Kinjo T, Hashioka H, Arakaki W, et al
    Impact of G29179T mutation on two commercial PCR assays for SARS-CoV-2 detection.
    J Virol Methods. 2023;314:114692.
    PubMed     Abstract available


  82. AOKI A, Adachi H, Mori Y, Ito M, et al
    A modified high-resolution melting-based assay (HRM) to identify the SARS-CoV-2 N501Y variant.
    J Virol Methods. 2023;314:114678.
    PubMed     Abstract available


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